TOOLS AND REAGENTS

IN VITRO CULTERING OF VERTEBRATE AIRWAY 
Airway organoids (AOs)

2D culture of proximal airway epithelia in Air-Liquid interface









Centrioles (gamma tubulin), Multi ciliated cells (Acetylated Tubulin) and Tight junctions (ZO-1)

 Centriole labelling: Confocal imaging and apical plane projection

                           

ADDITIONAL MODEL SYSTEMS IN THE LAB

Human primary epithelial cell cultures
       Primary culture of human cornea epithelial cells


Drosophila melanogaster
     Adult and pupa stages

Other 2D and 3D model systems:
     Polarized MDCK cells in 2D
     Polarized MDCK cells in 3D

TOOLS

    -QUANTITATIVE BIOLOGY
Centriole Co-ocurrence analysis

Centriole density analysis

Polarity vector analysis